Global Nitrilase Market Strategic Research Report

Scope of the Report

The global Nitrilase market size is predicted to grow from US$ 52.09 million in 2025 to US$ 88.38 million in 2032; it is expected to grow at a CAGR of 7.9% from 2026 to 2032.

In 2025, global nitrilase sales volume was approximately 15 tons, with an average global market price of about USD 3,550/kg. The gross margin of major manufacturers in the industry was approximately 45%–65%.

Nitrilase is a type of biocatalytic enzyme that directly hydrolyzes the —CN group in nitrile compounds to produce carboxylic acids and ammonia, and is an important category within nitrile-converting enzymes. Compared with conventional strong acid, strong alkali, or high-temperature hydrolysis processes, nitrilase enables milder reaction conditions and can usually operate in aqueous systems under neutral, weakly acidic, or weakly alkaline conditions. It offers advantages such as high selectivity, fewer side reactions, and lower environmental burden. Due to differences in enzyme source and engineering modification, different nitrilases vary significantly in substrate scope, chiral selectivity, regioselectivity, thermal stability, and pH tolerance. Commercial products are generally supplied in forms such as free enzyme preparation, immobilized enzyme preparation, and whole-cell catalyst.

The upstream of nitrilase mainly includes microbial strain resources, gene sequences, expression vectors, fermentation media, enzyme engineering technologies, immobilization carriers, and enzyme formulation excipients. The midstream involves enzyme screening, directed evolution, fermentation expression, purification, immobilization treatment, and process scale-up. Downstream applications are mainly concentrated in pharmaceutical biocatalysis, agrochemical intermediate, specialty fine chemical, and environmental remediation. In pharmaceutical and CDMO applications, nitrilase can be used for the green synthesis of chiral carboxylic acids, aromatic carboxylic acids, heterocyclic carboxylic acids, and other API intermediates. In agrochemical and specialty fine chemical applications, it is used for selective transformation of nitrile-containing substrates, replacing part of traditional chemical hydrolysis routes. In environmental remediation, nitrilase can be used for nitrile wastewater treatment and degradation of nitrile-containing pollutants.

Global key Nitrilase players cover Codexis, Almac Group, Prozomix, Cambrex, c-LEcta, etc.

Key Questions Addressed in this Report

What is the 10-year outlook for the global Nitrilase market?

What factors are driving Nitrilase market growth, globally and by region?

Which technologies are poised for the fastest growth by market and region?

How do Nitrilase market opportunities vary by end market size?

How does Nitrilase break out by Type, by Application?

This report presents a comprehensive overview of the global Nitrilase market, covering market size and forecast, segmentation by product type and application, competitive landscape, leading players and regional and country-level outlook.

Segment by Type

• Aliphatic Nitrilase
• Aromatic Nitrilase
• Heterocyclic Nitrilase

Segment by Catalytic Selectivity

• General Hydrolysis
• Chiral Selectivity
• Regioselectivity

Segment by Form

• Free Enzyme Preparation
• Immobilized Enzyme Preparation
• Whole-cell Catalyst

Segment by Application

• Pharmaceutical Biocatalysis
• Agrochemical Intermediate
• Specialty Fine Chemical
• Environmental Remediation
• Other

Who Can Use This Report?

This report is written for decision-makers who need a clear, data-backed view of the global Nitrilase market:

• Manufacturers, suppliers and solution providers benchmarking their position and planning product, capacity and go-to-market strategy
• Distributors, channel partners and end users in Pharmaceutical Biocatalysis, Agrochemical Intermediate, Specialty Fine Chemical evaluating demand and sourcing options
• Investors, financial analysts and consultants assessing growth opportunities, competitive dynamics and M&A potential
• Government agencies, industry associations and research institutions tracking industry developments and policy impact